Aggrecan expression
WebSep 29, 2016 · RUNX-2 is a major transcription factor regulating aggrecan loss via the regulation of ADAMTS-4/5 gene expression [29,30] while SOX-9 acts as a master regulator of aggrecanases at both the transcriptional and posttranscriptional levels [31,32,33]. This distinction may explain why some markers appeared to be stronger at the protein level … WebMar 24, 2024 · The expression levels of genes encoding collagen II, aggrecan, Sox9, and Osteocalcin were quantified using RT-PCR on a Real-time PCR thermocycler (ABI Stepone, USA). The sequence of primers (Sinaclon, Iran) that we used in real-time PCR was as Table 2. Table 2 Primer sequences for RT-PCR Full size table
Aggrecan expression
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WebMar 2, 2024 · (D) Immunostaining analysis showing the expression of fatty acid binding protein 4 (FABP4), osteocalcin and aggrecan in adipogenic, osteogenic, and chondrogenic induced cells, respectively. No staining has been seen for FABP4 in undifferentiated (UD) controls, but weak staining observed for osteocalcin and aggrecan in UD controls. Aggrecan is a proteoglycan, or a protein modified with large carbohydrates; the human form of the protein is 2316 amino acids long and can be expressed in multiple isoforms due to alternative splicing. Aggrecan was named for its ability to form large aggregates in the cartilage tissue (a large aggregating … See more Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. This gene is a member of the See more Aggrecan is a high molecular weight (1x10 < M < 3x10 ) proteoglycan. It exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate glycosaminoglycan … See more The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. The linker domain between the N-terminal globular domains, called the interglobular domain, is highly … See more • Aggrecan at the U.S. National Library of Medicine Medical Subject Headings (MeSH) • Human ACAN genome location and See more Aggrecan is a critical component for cartilage structure and the function of joints. Functionally, the G1 domain interacts with hyaluronic acid and link protein, forming stable ternary complexes in the extracellular matrix. … See more • Watanabe H, Yamada Y, Kimata K (1998). "Roles of aggrecan, a large chondroitin sulfate proteoglycan, in cartilage structure and function" See more
WebExpression of type II collagen or aggrecan was not detected immunohistochemically in skin or bone. These results demonstrate that human articular chondrocytes can be characterized in culture, by the combined application of PCR and immunohistochemistry. WebSep 29, 2016 · RUNX-2 is a major transcription factor regulating aggrecan loss via the regulation of ADAMTS-4/5 gene expression [29,30] while SOX-9 acts as a master …
WebExpression of constitutively active (CA)-PI3K significantly induced aggrecan promoter activity. We observed that PI3K maintained Sox9 gene expression and activity: inhibition of PI3K/AKT resulted in decreased Sox9 expression, lowered promoter activity, and mediated a reduction in Sox9 transcriptional activity. WebNov 15, 2024 · Previous research revealed that an imbalance in the extracellular matrix (ECM) via abnormal hypoxia-inducible factor-1alpha (HIF-1α) expression in nucleus pulposus (NP) cells was one of the key factors in the pathogenesis of intervertebral disc degeneration (IDD).
WebMar 14, 2014 · Immunohistochemical and Western Blot analyses revealed an increased expression of collagen type II and aggrecan in heat-shocked pellets than non heat-shocked pellets on Day 17 of chondrogenesis. In addition, HS also upregulated the expression of collagen type I and X as well as heat shock protein 70 on Day 17 and 24 …
WebApr 13, 2024 · To select the KLF3-AS1 expression-related genes, Pearson’s correlation coefficient was used to analyze the expression correlation based on GSE70362 and GSE124272 (∣r∣ > 0.5, p < 0.05). Six gene (DDHD2, LINC00342, ZBTB20, SUN1, NR3C1, and ZFP90) expressions were positively correlated with KLF3-AS1 expression, and 3 … brass monkey restaurant and barWebDownload scientific diagram Western blot analysis of COL2, aggrecan, and MMP-3 in each group. (a) Protein expression levels of COL2, aggrecan, and MMP-3. (b) Semiquantitative analysis of COL2. brass monkeys band green bayWebThe localizations of aggrecan and Col-II, as well as the SUMOylation modification of these proteins were analyzed using immunofluorescence and immunoprecipitation assays, respectively.Results: Our results showed that a proinflammatory cytokine interleukin-1β induced the OA model and desumoylation of aggrecan and collagen type II because the ... brass monkey strain leaflyWebFeb 28, 2024 · This suggests that eNOS deficiency may elevate aggrecan expression, which promotes apoptosis in VSMC, thereby contributing to atherosclerosis progression. These results may facilitate the development of novel approaches for improving the diagnosis or treatment of atherosclerosis. brass monkey sayingWebMay 4, 2024 · Aggrecan expression is seen from embryonic stages and increases with age; it is widespread, even in the normal adult retina, particularly in the synaptic layers … brass monkey song beastie boysWebApr 10, 2024 · The expressions of DYRK1A and its downstream signal epidermal growth factor receptor (EGFR) were detected in the cartilage of adult wild-type mice with … brass monkey strainWebMar 1, 2002 · Aggrecan is a multimodular molecule expressed by chondrocytes. Its core protein is composed of three globular domains (G1, G2, and G3) and a large extended … brass monkey the shore